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Blackwell Synergy - Marine Mammal Sci, Volume 11 Issue 3 Page 344-361, July 1995 (Article Abstract)
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Issue online:
26 Aug 2006
Received: 29 March 1994 Accepted: 4 January 1995
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Article Abstract
Marine Mammal Science
Volume 11 Issue 3 Page 344-361, July 1995
To cite this article:
Yoshiyuki Ukishima, Yumiko Sakane, Akira Fukuda, Shiro Wada, Shoji Okada (1995)
IDENTIFICATION OF WHALE SPECIES BY LIQUID CHROMATOGRAPHIC ANALYSIS OF SARCOPLASMIC PROTEINS
Marine Mammal Science 11 (3)
, 344–361
doi:10.1111/j.1748-7692.1995.tb00290.x
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AbstractIDENTIFICATION OF WHALE SPECIES BY LIQUID CHROMATOGRAPHIC ANALYSIS OF SARCOPLASMIC PROTEINSYoshiyuki Ukishima11Shizuoka Prefectural Fujieda Public Health Center, 2-2-25 Okadeyama, Fujieda 426, Japan, Yumiko Sakane11Shizuoka Prefectural Fujieda Public Health Center, 2-2-25 Okadeyama, Fujieda 426, Japan, Akira Fukuda11Shizuoka Prefectural Fujieda Public Health Center, 2-2-25 Okadeyama, Fujieda 426, Japan, Shiro Wada22National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa-ku, Yokohama 236, Japan, Shoji Okada33University of Shizuoka School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka 422, Japan 1Shizuoka Prefectural Fujieda Public Health Center, 2-2-25 Okadeyama, Fujieda 426, Japan 2National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa-ku, Yokohama 236, Japan 3University of Shizuoka School of Pharmaceutical Sciences, 52-1 Yada, Shizuoka 422, Japan Abstract Liquid chromatography (LC) was applied to identify whale species by analyzing water-soluble sarcoplasmic proteins in skeletal muscles. Twenty-five samples from four baleen whale species (fin whale, sei whale, Bryde's whale, and minke whale) and eight toothed whale species (sperm whale, Baird's beaked whale, short-finned pilot whale, Dall's porpoise, northern right whale dolphin, Pacific white-sided dolphin, common dolphin, and striped dolphin) were analyzed. Water-soluble sarcoplasmic proteins were extracted from each sample and analyzed using a UV-VIS spectrophotometric detector at 280 nm and a pho-todiode array detector. The chromatographic profiles of each sample showed distinctive qualitative and quantitative characteristics for each whale species, making species identification possible. A photodiode array detector was useful for further accurate identification of whale species by obtaining the absorption spectra of separated protein peaks. These results suggest that the LC method is readily applicable to rapid, simple, and reliable identification of whale species.
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SearchInSynergyCrossRefBy keywordsIdentification of whale speciessarcoplasmic proteinsliquid chromatographic profilesrelative retention time%total areaabsorption spectraBy authorYoshiyuki UkishimaYumiko SakaneAkira FukudaShiro WadaShoji Okada
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